表皮生长因子对牦牛卵丘细胞低氧诱导因子-1α表达的影响及与凋亡的关联性分析

doi:10.11843/j.issn.0366-6964.2016.06.010

Abstract

Abstract:

The present study was carried out to verify whether EGF inhibited the apoptosis of cumulus cells of yak by regulating the expression of HIF-1α.The cumulus cells of yak were cultured in vitro with the supplement of different concentration of EGF.The expression levels of HIF-1α，Bax and Bcl-2 in different groups were detected by qRT-PCR and immunofluorescence.TUNEL was used to evaluate apoptosis rate.Results showed that：(1) After the addition of different concentration of EGF to the cumulus cells，there would be found a declined mRNA relative expression of HIF-1α and Bax and an increased mRNA relative expression of Bcl-2，with a characteristic manner of concentration-dependent.When the concentration of EGF was as high as 50 ng•mL-1，the mRNA relative expressions of HIF-1α and Bax were the lowest，while that of Bcl-2 was the highest.(2) The protein relative expressions of HIF-1α and Bax were decreased and that of Bcl-2 was increased，with a characteristic manner of concentration-dependent.When the concentration of EGF was as high as 50 ng•mL-1，the protein relative expressions of HIF-1α and Bax were the lowest，while that of Bcl-2 was the highest.(3) Apoptosis detection shown that the apoptosis rate from control group was the highest.It was extremely declined when the concentration of EGF was 25 ng•mL-1 (P＜0.05)，and down to its lowest level when EGF was 50 ng•mL-1 (P＜0.05)，while rose again when EGF was continues to increase.It is concluded from this study that EGF can inhibit the apoptosis of cumulus cells by regulating HIF-1α and may be related to mitochondrial Bax and Bcl-2 apoptotic pathway.

CLC Number:

S823.8+5.2

ZHANG Yi-fu，PAN Yang-yang，WEN Ze-xing，YU Si-jiu. The Effect of Epidermal Growth Factor on the Expression of Hypoxia Inducible Factor-1α in Cumulus Cells of Yak (Bos grunniens) and Its Correlation Analysis with Apoptosis[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(6): 1154-1161.

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The Effect of Epidermal Growth Factor on the Expression of Hypoxia Inducible Factor-1α in Cumulus Cells of Yak (Bos grunniens) and Its Correlation Analysis with Apoptosis

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